Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and …
PrimerBank: a PCR primer database for quantitative gene expression ...
Optimization of primer sequences for polymerase chain reaction (PCR) and quantitative PCR (qPCR) and reaction conditions remains an experimental challenge. We have developed a resource, …
Primer designing tool - National Center for Biotechnology Information
The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction.
Fundamentals of Cellular Calcium Signaling: A Primer - PMC
This primer discusses basic principles and mechanisms underlying cellular Ca 2+ signaling and Ca 2+ homeostasis. Consequently, we have cited some historical articles in addition to more recent findings.
Methods to Disseminate Research: A Primer - PMC
Decisions in healthcare, policy, education and other fields use data to drive action and change (Ruiz et al., 2022). Hence, communicating research findings is essential to ensure knowledge acquired can …
Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers. The longest sequence is also …
Molecular basis for the initiation of DNA primer synthesis
The structural and mechanistic studies presented here establish how Prim-Pol proteins instigate primer synthesis, revealing the requisite molecular determinants for primer synthesis within the catalytic …
Primer-BLAST: A tool to design target-specific primers for polymerase ...
Users can design new primer pairs by entering the DNA template alone or they can design one primer by entering the template plus the other pre-existing primer. Primer-BLAST can check the specificity …
Primer design for RT-PCR - PubMed
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency …
The Glucocorticoid Taper: A Primer for the Clinicians - PubMed
Affiliations 1 Department of Endocrinology, Fortis Hospital, Mohali, Punjab, India. 2 Department of Endocrinology, Sher-i-Kashmir Institute of Medical Sciences ...